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分類:導師信息 來源:中國考研網 2015-07-13 相關院校:華中農業大學
基本信息
姓名:金雙俠 出生年月:1979.2
性別:男 碩/博導:碩導
民族:漢 開設課程:植物生物技術,作物生產實習,育種學實習
職稱:副教授 研究方向:棉花生物技術;細胞質基因組及生物技術
學位:博士
聯系方式辦公電話:027-87283955
電子郵件:jinsx1979@gmail.com
個人簡介華中農業大學植物科學技術學院作物遺傳育種專業博士,副教授,碩士生導師。1997-2001,華中農業大學農學系農學專業就讀;2001-2006,華中農業大學植物科學技術學院攻讀碩士、博士學位,獲農學博士學位,指導教師均為張獻龍教授.2007.10-2012.4在美國UniversityofCentralFlorida,HenryDaniell教授指導下進行博士后研究,研究方向為植物葉綠體轉化。研究領域主要涉及棉花細胞、組織培養、棉花轉基因等。目前從事棉花抗病,蟲及逆境基因的克隆及高效表達載體(葉綠體轉化表達體系)的開發及轉基因研究
科研項目湖北省自然科學基金重點項目(杰青項目)主持,2015.1-2016.12
國家自然科學基金煙粉虱與棉花互作的分子機理研究主持,2014-2017
國家自然科學基金植物凝集素基因在棉花中的功能分析,主持,2012-2015
新疆建設兵團博士資金項目轉基因抗病蟲、抗逆聚合雜交棉新組合選育技術研究,主持,2007-2009
國家自然科學基金棉花外源基因葉綠體轉化體系的建立及其應用(30771368),主持,2008-2010
教育部博士點新教師基金以gfp基因為報告基因監測轉基因棉花花粉漂移(20070504087),主持,2008-2010
國家自然科學基金重大國際合作項目Developmentofstrongpest-resistancecottonviatransformingplastidanditsapplicationinbreeding,主要參加者,2009-2011
國家自然科學基金利用基因誘捕構建棉花功能基因組研究突變群體,主要參加,2005-2007
發明專利及獲獎情況獲獎情況:
湖北省優秀博士學位論文(第10批)獲獎者,2008年
湖北省優秀本科論文指導獎,2008年。本人指導的2007界本科生張碧瑤,其論文:新疆棉花高體細胞胚胎發生能力基因型的篩選,獲得湖北省優秀本科學位論文。
“棉花分子育種技術體系建立與應用”獲2008年湖北省技術發明二等獎
獲第十、十一屆湖北省自然科學優秀論文一等獎2次
申報兩項發明專利:
超聲波輔助農桿菌轉化棉花胚芽的方法,專利申請號:200710063650.0
一種分離棉花葉綠體DNA的方法,專利申請號:200710052665.7
發表的論文及著作[1]JinShuanxia,Singh,Dolendro,LiLebin,Zhang,XianlongandDaniell,Henry.EngineeredchloroplastdsRNAsilencescytochromep450monooxygenase,V-ATPaseandchitinsynthasesgenesintheinsectgutanddisruptsHelicoverpaarmigeralarvaldevelopmentandpupation.PlantBiotechnologyJournal,2015,online,doi:10.1111/pbi.12355.
[2]LebinLi§•YiZhu§•ShuangxiaJin*•XianlongZhang.PyramidingBtgenesforincreasingresistanceofcottontotwomajorlepidopteranPests:SpodopteralituraandHeliothisarmigera.ActaPhysiologiaePlantarum,2014,36(10),2717-2727(SCI,IF:1.5)
[3]ShuangxiaJinandHenryDaniell.Expressionofγ-tocopherolmethyltransferaseinchloroplastsresultsinmassiveproliferationoftheinnerenvelopemembraneanddecreasessusceptibilitytosaltandmetal-inducedoxidativestressbyreducingreactiveoxygenspecies.PlantBiotechnologyJournal,2014(12),1274-1285.(SCI,IF:6.3)
[4]KwangChulKwon*,DheerajVerma*,ShuangxiaJin*,HenryDaniell.Exportofproteinsfromchloroplastsinducedbyreactiveoxygenspeciesduringbioticandabioticstress.PLOSONE,2013,8(6):e67106.(*并列第一).(SCI,IF:3.7)
[5]DheerajVerma,ShuangxiaJin,AndersonKanagaraj,NameirakpamD.Singh1,JaiyanthDaniel1,PappachanEKolattukudy1,MichaelMiller2andHenryDaniell1*ExpressionofFungalCutinaseandSwollenininTobaccoChloroplastsRevealsNovelEnzymeFunctionsand/orSubstrates.PLOSONE,2013;8(2):e57187(SCI,IF:3.7)
[6]GuanzeLiu,XuelinLi,ShuangxiaJin,XuyanLiu,LongfuZhu,YichunNie,XianlongZhang.OverexpressionofRiceNACGeneSNAC1ImprovesDroughtandSaltTolerancebyEnhancingRootDevelopmentandReducingTranspirationRateinTransgenicCotton.2014.PLoSOne,e86895
[7]WangY,JinS,WangM,ZhuL,ZhangX(2013)IsolationandCharacterizationofaConservedDomainintheEremophyteH+-PPaseFamily.PLoSONE8(7):e70099.(SCIIF3.7)
[8]ShuangxiaJin,XianlongZhang1,HenryDaniell.Pinelliaternataagglutininexpressioninchloroplastsconfersbroadspectrumresistanceagainstaphid,whitefly,lepidopteraninsects,bacterialandviralpathogens.PlantBiotechnologyJournal2012,10(3):313-327(CoverStory,IF6.3).
[9]JinSX,liuGZ,ZhuHG,YangXYandZhangXL.TransformationofUplandCotton(GossypiumhirsutumL.)withgfpGeneasaVisualMarker.JournalofIntegrativeAgriculture,2012,11(6):910-919,(SCI)
[10]LiuG,JinS,LiuX,TanJ,YangX,ZhangX(2012)OverexpressionofArabidopsiscyclinD2;1incottonresultsinleafcurlingandotherplantarchitecturalmodifications.PlantCell,TissueOrganCult110(2):261273(SCI,IF:3.6)
[11]ShuangxiaJin,AndersonKanagaraj,DheerajVerma,TheoLange,HenryDaniell.ReleaseofHormonesfromConjugates:ChloroplastExpressionofß-glucosidaseResultsinElevatedPhytohormoneLevelsAssociatedwithSignificantIncreaseinBiomassandProtectionfromAphidsorWhitefliesConferredbySucroseEsters.PlantPhysiol,2011,155,222-235.(SCI,IF:6.5)
[12]Seung-BumLee,BaichuanLi,ShuangxiaJinandHenryDaniell.Expressionandcharacterizationof antimicrobialpeptidesRetrocyclin-101andProtegrin-1inchloroplaststopreventsexualtransmissionofdiseases.PlantBiotechnologyJournal.2011,9.100-115.(SCI,IF:6.3)
[13]VermaD,KanagarajA,JinSX,SinghN,KolattukudyP,DaniellH.Chloroplast-derivedenzymecocktailshydrolyselignocellulosicbiomassandreleasefermentablesugars.PlantBiotechnologyJournal,2010,8:332-350 (SCI,IF:6.3).
[14]JinSX,ZhangXL,ZhuHG,ZhouZL,NieYC.Detectionofsomaclonalvariationofcotton(Gossypiumhirsutum)throughRAPD,SSRmarkers,cytogeneticandflowcytometeranalysis.PlantCellReports,2008,27:1303–1316.(SCI)
[15]ShuangxiaJin,XianlongZhang,etal,Identificationofanovelelitegenotypeforinvitrocultureandgenetictransformationofcotton.BiologiaPlantarum,200650(4):519-524.(SCI)
[16]ShuangxiaJin,XianlongZhang(2006)Anefficientgraftingsystemfortransgenicplantrecoveryincotton(GossypiumhirsutumL.).PlantCell,TissueandOrganCulture,85(5):181-185.(SCI)
[17]ShuangxiaJin,XianlongZhang(2005)FactorsaffectingstabletransformationandplantregenerationduringtransformingembryogeniccallusofUplandcotton(GossypiumhirsutumL.)viaAgrobacteriumtumefaciens,PlantCell,TissueandOrganCulture,81(2):229-237.(SCI)
[18]ShuangxiaJin,XianlongZhang(2005)FunctionAnalysisofPromoterTrappingSystemafterInsertingCotton(GossypiumhirsutumL.)Genome.JGENETGENOMICS(ActaGeneticaSinca),32(12):1266-1274.
[19]XianlongZhangandShuangxiaJin(2007)TransgenicCotton:AnOverview(invitedreview).TransgenicPlantJournal,2007,1(1),150-162.
[20]FangDing,ShuangxiaJin,NiHong,YunZhong,QingCao,GanjunYiandGuopingWang.2008.Vitrification–cryopreservation,anefficientmethodforeliminatingCandidatusLiberobacterasiaticus,thecitrusHuanglongbingpathogen,frominvitroadultshoottips.PlantCellReports,27(2),241-250(SCI)
[21]SunYQ,ZhangXL,JinSX.Productionandcharacterizationofsomatichybridsbetweenuplandcotton(Gossypiumhirsutum)andwildcotton(G.klotzschianumAnderss)viaelectrofusion.TheorApplGenet,2004,109:472-479(SCI)
[22]SunYQ,ZhangXL,JinSXSomaticembryogenesisandplantregenerationinwildcotton(GossypiumklotzschianumAnderss).PlantCellTissureOrganCult,2003,75:247-253(SCI)
[23]WuJH,ZhangXL,JinSX.FactorsaffectingsomaticembryogenesisandplantregenerationfromarangeofrecalcitrantgenetypesofChinesecotton.InvitroCellDevBiol-Plant,2004,40:371-375(SCI)
[24]GUOX,JINS,ZHANGX(2007)Agrobacterium-mediatedtransformationofCry1C,Cry2AandCry9CgenesintoGossypiumhirsutumandplantregeneration.BiologiaPlantarum,51(2):242-248(SCI)
[25]XiyanYang,XianlongZhang,ShuangxiaJin,LiliFu,LinggangWangProductionandcharacterizationofasymmetrichybridsbetweenuplandcottonCoker201(Gossypiumhirsutum)andwildcotton(GossypiumklozschianumAnderss),PlantCellTissureOrganCult,2007,89:225–235(SCI)
[26]YangX,GuoX,ZhangX,NieYandJinS.PlantregenerationfromGossypiumdavidsoniiprotoplastsviasomaticembryogenesis.BIOLOGIAPLANTARUM,51(3):533-537,2007(SCI)
[27]XianlongZhang,ShuangxiaJin,DiqiuLiu,ZhongxuLin,FanchangZeng,LongfuZhu,LiliTu,XiaopingGuo.CottonBiotechnology:ChallengetheFutureforCottonImprovement,In:BiotechnologyinCropImprovement,GPRao(ed.),StudiumPress,LLC,Houstan,Texas,USA.Pp.241-301
[28]LiliTu,XianlongZhang,DiqiuLiu,ShuangxiaJin,JinglinCao,LongfuZhu,FenglinDeng,JiafuTan,CunbinZhang,2007,SuitableinternalcontrolgenesforqRT-PCRnormalizationincottonfiberdevelopmentandsomaticembryogenesis,ChineseScienceBulletin52:3110-3117(SCI)
[29]周小鳳,金雙俠,李保成,余渝,寧新柱,王旭文.初步研究不同激素對海島棉體細胞胚胎發生的影響.中國農學通報2009,25(11):78-83
[30]朱華國,涂禮莉,金雙俠,徐理,譚家福,鄧鋒林,張獻龍.棉花細胞初始脫分化的基因差異表達分析.科學通報,2008,53(20),2483-492(SCI)
[31]曹景林張獻龍金雙俠楊細燕朱華國付莉莉.棉花高效體細胞胚發生及同步控制培養體系研究.作物學報,2008,34(2):224−231
[32]謝德意,金雙俠,郭小平,張獻龍.長江和黃河流域棉區棉花品種體細胞胚胎發生和植株再生能力研究,作物學報,2007,33(3):394-400
[33]謝德意,金雙俠,郭小平,張獻龍.棉花ipt基因遺傳轉化效率及優化成苗技術研究,作物學報,2007,33(5)
[34]金雙俠,韓杰,劉小云,劉冠澤,王一嫻,唐文鑫,張獻龍.SDS-蛋白酶法分離棉花cpDNA及psbA基因啟動子、終止子克隆.分子植物育種,2007,5,683-689.
[35]周小鳳,張碧瑤,劉冠澤,高巍,余渝,鄧福軍,李保成,孔憲輝,張獻龍,金雙俠*.新疆棉花高體細胞胚胎發生能力基因型的篩選,分子植物育種,2007,6,1-8(通訊作者).
[36]張獻龍,孫玉強,吳家和,金雙俠,聶以春,郭小平,2004,棉花細胞工程及新種質創造,棉花學報,16(6):368-373
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