華中農(nóng)業(yè)大學(xué)

1985-1999 As an assistantin 1985, I engaged in propagation and extending the citrus fine varieties and virus-free plants, won the first prize and the three prizes of the national science and technology progress by Ministry of Agriculture and Education Committee for “Propagation and popularization of satsuma mandarin Guoqing No. 1 and No. 4”in 1988 and in 1994. During studying for Master Degree, I explored and improved the method of the shoot tip grafting for elimination the pathogen of Huanglongbing. During studying for PhD, I performed the selecting the high content of medicinal components in Ginkgo biloba using cell suspension culture, and developed the antibodies of hapten quercetin.

2000-2002Obtained the fellowship of senior visiting scholar, engaging in the research of CP gene transformation of papaya in Japan international researching center for two years in JRCAS.

2003-2006Presided over the projects of the Ministry and Province about “Detection of papaya ringspot virus using the coat protein gene probe" and "Citrus virus-free micro-propagation and molecular detection “.

2006-2008 Presided over the National Natural Science Foundation (No.30571277) "Function in RNA silencing path of siRNA in the papaya protoplasts and virus inhibitor pathway ".

2008-2010 Presided over the National Natural Science Foundation "Research on ihpRNA mediated papaya ringspot virus resistance and defense response regulator of the VIGS “(No.30771477).

2010-2012Participated in the projects of "Study on citrus germplasm research and genetic improvement" presided by professor Deng xiuxin; and "Citrus model cultivation and storage technology" presided by professor Yi Hualin; participated in Hubei province key project "The application of poly-r-glutamic acid in the fruit tree" (ZDNDD17)”.

2012-2014 Having participated in the project of the Ministry of Agriculture of China about “Comprehensive technology research and demonstration of controlling citrus yellow shoot disease and citrus canker”, and responsible for one of the sub-topics project (20100306701) since 2010. Carried out the research of structural biology related protein of Huanglongbing pathogen in 2011. Presided over National Natural Science Foundation “Study on the molecular mechanism of controlling the key enzymes basing on fatty acid metabolism of Huanglongbing in citrus” (No.31272146).

American Society for Horticultural Science (ASHS) member 2014, Citrus association member of China.

Publications

(1)Jiang L, Gao Z, Li Y, Wang S, Dong Y*.Crystal structures and kinetic properties of enoyl-acyl carrier protein reductase I from Candidatus Liberibacter asiaticus.Protein Sci. 2014,23(4):366-377. doi: 10.1002/pro.2418.Epub 2014 Feb 12.

(2)Pan LJ, JIANG L*. Identification and expression of the WRKY transcription factors of Carica papayain Response to abiotic and biotic stresses. Mol Biol Rep (2014) 41:1215–1225, DOI 10.1007/s11033-013-2966-8

(3) L. JIANG, ZQ GAO, YH. DONG. Crystal structure of FabI from Candidatus Liberibacter asiaticus, Crystal structure of FabI-NAD complex from Candidatus Liberibacter asiaticus. Structures PDB IDs [4NK4, 4NK5] and RCSB IDs [RCSB083303, RCSB083304] be released on 2014-02-12, they have been electronically published in PDB.
TITLE: Crystal structure of FabI-NAD complex from Candidatus Liberibacter　asiaticus. Protein science. 2014,23(4):366-377

(4) LIU Fandan, LIU liu, GAO Jiulin, XIE Jingchang, JIANG Ling*. Studing on improving thequality using poly-γ-glutamic acid in grape of Fujiminori grapevines. South China Fruits. 2014, 43 (1):23-27

(5) Jiang L*. Pan LJ. Identification and expression of C2H2 transcription factor genes in Carica papaya under abiotic and biotic stresses. Molecular Biology Reports, 2012, 39(6):7105-7115. DOI: 10.1007/s11033-012-1542-y

(6)QIAO Fen, QI Peng-zhi, FU Hui, ZHANG Peng, WU Shan, JIANG Ling*. Eradicating double infected pathogen of CTV and CTLV in Citrus. Journal o f Huazhong Agricultural University. 2011, 30(4):416-421

(7)ZHANG Fan, JIANG Ling*. Virus-resistance of Papaya Ringspot Virus mediated by double-strand RNA. Plant Science Journal, 2011，29(3): 385-391

(8)JIANG L*. Wang J. Liu Z. Wang L. Liu GC, Zhang F. Silencing induced by inverted repeat constructs in protoplasts of Nicotiana benthamiana. Plant Cell, Tissue and Organ Culture.2010, 100:139-148

(9) YU Shan-baoa,ZHANG Hong-yana,CHEN Shou-wenb, JIANG Ling*. Effect of poly-γ-glutamic acid on the yield and fruit quality of strawberry. Hubei Agricultural Sciences, 2010, 49(1):1638-1641

(10) JIANG Ling*, Qin Chang-ping, Fu Hui. Quick construction of inverted repeat vector of Papaya ringspot virus CP gene by GatewayTMSystem.Journal of Agricultural Biotechnology 2008, 16 (3): 526~529

(11) JIANG Ling*, ZHANG Hong-yan, ZHANG Min-tao, LIU Xiao-mei, FU Hui. Detection of Papaya ringspot virus (PRSV) by cDNA probeslabelled with digoxgenin labelling and alkPhos direct labelling. Journal of Agricultural Biotechnology 2007, 15 (3): 496~502

PATENT

JIANG Ling and Lu Minyu

Title: Pre-treatment of scion facilitate the elimination of the complex pathogen in citrus

Number of patent: 201410132037X