四川大學生命科學學院導師信息介紹：鮑錦庫

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姓名：鮑錦庫　性別：男

職稱：教授　學院：生命科學學院

職務：

中國生物化學與分子生物學會糖復合物專業(yè)委員會委員、常務委員

中國生物化學與分子生物學會生物化學與分子生物學教學專業(yè)委員會委員、常務委員

中國生物工程學會糖工程專業(yè)委員會委員

國際凝集素委員會委員

聯(lián)系方式：

辦公電話：028-85415171

電子郵件：baojinku@scu.edu.cn

學位簡歷：

1987.09-1991.07：浙江大學生物系生物化學與微生物學專業(yè)，本科

1991.09 –1994.07：四川大生物系生物化學專業(yè)，理學碩士

2000.09 –2003.12：四川大學生命科學學院，理學博士

工作簡歷：

1994.07–1996.07：四川大學生命科學學院，助教

1994.09 –1995.09：

在中國農(nóng)科院植保所植物病蟲害生物學國家重點實驗室和中國農(nóng)科院畜牧研究所客座研究

1996.07–1998.07：

四川大學生命科學學院，講師

1998.07–2005.07：

四川大學生命科學學院副教授

2005.07-2006.07：

四川大學生命科學學院教授博士生導師

2006.07 –至今：

四川大學生命科學學院教授，博士生導師。

個人科研工作簡介

1）研究方向及主要學術成果：

立足我國豐富的天然藥用生物資源寶庫，發(fā)揮在天然藥物研發(fā)的資源優(yōu)勢，圍繞艾滋病等傳染性疾病、臨床常見惡性腫瘤等疾病及重大新藥創(chuàng)制等國家戰(zhàn)略需求，以具有特異糖結合能力的非免疫來源的凝集素為主線，集成現(xiàn)代生物技術手段，進行具有自主知識產(chǎn)權、抗病毒和抗腫瘤活性的凝集素等活性大分子的篩選、規(guī)模制備、基因克隆與蛋白序列、重組蛋白表達、生物活性及其基于腫瘤調(diào)控網(wǎng)絡的分子機制、基于結構的分子設計和改造以及蛋白多肽數(shù)據(jù)庫的構建和利用轉基因植物大量獲得活性蛋白等系統(tǒng)研究。發(fā)揮生命科學、醫(yī)學和信息科學等學科特點及學科交叉優(yōu)勢，引入系統(tǒng)生物學整體性、信息化和網(wǎng)絡調(diào)控的研究策略和理念，集成生物化學與分子生物學、細胞生物學、分子藥理學、組學和系統(tǒng)生物學等技術方法，系統(tǒng)研究中草藥蛋白多肽抗腫瘤分子機制及其潛在靶標，拓辟了“凝集素探針-腫瘤細胞程序性死亡相關蛋白-miRNA相互調(diào)控網(wǎng)絡”研究新方向，為基于中藥源活性蛋白多肽為先導或候選化合物的功能機制研究提供示范性的新思路并為源頭創(chuàng)新藥物研發(fā)提供通用模式。

2）科研項目：

國家自然科學基金項目：

1、寡糖鏈、氨基酸側鏈基團與黃精凝集素生物學活性研究(批準號:39770178),1998 -2000,項目負責人

2、黃精凝集素結構與生物學活性關系研究（批準號：30000032),2001－2003,項目負責人

3、定點突變研究黃精凝集素II結構與功能關系（批準號：30270331),2003－2005，項目負責人

4、重組和突變抗艾滋病毒黃精凝集素II及其復合物晶體結構研究(批準號:30670469),2007－2009，項目負責人

5、黃精凝集素誘導腫瘤細胞自噬和凋亡的分子機制研究(批準號:30970643),2010-2012,項目負責人

6、凝集素誘導腫瘤細胞程序性死亡的分子機制(批準號:81173093)，2012-2015, 項目負責人

7、十八屆國際凝集素會議18th International Lectin Meeting, July, 1999, U.K），英國，項目負責人

8、第十九屆國際凝集素會議（19th International Lectin Meeting, March, 2001, Brazil), 巴西，項目負責人

9、第二十屆國際凝集素會議（20th International Lectin Meeting, May,2002，Denmark), 丹麥，項目負責人

10、第二十一屆國際凝集素會議(21th International Lectin Meeting, May, 2004, Japan),日本,項目負責人

國家重大科技專項-轉基因生物新品種培育：

“西南地區(qū)抗水稻脅迫因子基因克隆及功能驗證”（2009ZX8009-072B）子課題負責人

國家高技術研究發(fā)展計劃（863計劃）：

番茄營養(yǎng)品質改良的基因工程技術研究(2007AA10Z122)，2007-2010，課題副組長

省部級課題：

1、中國農(nóng)科院植物保護研究所植物病蟲害生物學國家重點實驗室開放課題：蔥蓮凝集素的基因克隆，2002，1-12，項目負責人

2、四川大學科技創(chuàng)新基金:我國傳統(tǒng)中草藥中具抗病毒活性的甘露糖結合蛋白的基因克隆與表達研究, 2004-2006，項目負責人

3、四川省杰出青年科技基金：轉植物凝集素基因培養(yǎng)抗蟲新品種（批準號：06ZQ026-035），2006－2008，項目負責人

4、四川省科技攻關項目：傳統(tǒng)中草藥中抗病毒抗腫瘤蛋白的分離、基因克隆表達及活性研究 (批準號：2006Z08－010),2006－2008，
項目負責人

3）發(fā)表論文：

2012年：

Li WW, Li J, Bao JK*. Microautophagy: lesser-known self-eating. Cellular and Molecular Life Sciences. 2011. Doi:

10.1007/s00018-011-0865-5 (IF=7.047)

Wang Y, Wang H, Liu Y, Li C, Qi P, Bao J*. Antihyperglycemic Effect of Ginsenoside Rh2 by Inducing Islet β-cell

Regeneration in Mice. Hormone and Metabolic Research. 2012. 44(1):33-40. (IF=2.414)

Xu HL, Li CY, He XM, Niu KQ, Peng H, Li WW, Zhou CC, Bao JK*. Molecular Modeling, Docking and Dynamic Simulation of GNA-related Lectins for Potential Prevention in Influenza Virus (H1N1). Journal of Molecular Modeling. 2012, 18(1):27-37. (IF 1.871)

Wang Y, Liu YD, Wang HL, Li CY, Qi P, Bao JK*. Agaricus bisporus lectins mediates islet β-cell proliferation through regulation of cell cycle proteins. Experimental Biology and Medicine. 2012, DOI: 10.1258 /ebm.2011.011251. (IF 2.954)

2011年

Liu JJ, Lin M, Yu JY, Liu B*, Bao JK*. Targeting apoptotic and autophagic pathways for cancer therapeutics. Cancer Letters. 2011. 300(2):105-114. (IF: 4.864) 總引次數(shù) 16 次，他引次數(shù) 12 次。

Wang SY, Yu QJ, Bao JK*, Liu B. Polygonatum cyrtonema lectin, a potential antineoplastic drug targeting programmed cell death pathways. Biochemical and Biophysical Research Communications. 2011. 406(4):497-500. (IF: 2.595) 總引次數(shù) 4 次，他引次數(shù) 2 次。

Li WW, Yu JY, Xu HL, Bao JK*. Concanavalin A: a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics. Biochemical and Biophysical Research Communications. 2011. 414(2):282-286. (IF: 2.595)

Li CY, Luo P, Liu JJ, Wang EQ, Li WW, Ding ZH, Mou L, Bao JK*. Recombinant expression of Polygonatum cyrtonema lectin with anti-viral, apoptosis-inducing activities and preliminary crystallization. Process Biochemistry. 2011. 46(2): 533-542. (IF: 2.648) 總引次數(shù) 2 次，他引次數(shù) 1 次。

Li CY, Wang EQ, Cheng Y, Bao JK*. Oridonin: An active diterpenoid targeting cell cycle arrest, apoptotic and autophagic pathways for cancer theraputics. The International Journal of Biochemistry & Cell Biology. 2011. 43(5):701-704. (IF: 4.956) 總引次數(shù) 4 次，他引次數(shù) 3 次。

Fu LL, Zhou CC, Yao S, Yu JY, Liu B, Bao JK*. Plant lectins: targeting programmed cell death pathways as antitumor agents. The International Journal of Biochemistry & Cell Biology. 2011. 43(10):1442-1449. (IF: 4.956) 總引次數(shù) 1 次，他引次數(shù) 1 次。

Yang Y, Xu HL, Zhang ZT, Liu JJ, Li WW, Ming H, Bao JK*. Characterization, molecular cloning, and in silico analysis of a novel mannose-binding lectin from Polygonatum odoratum (Mill.) with anti-HSV-II and apoptosis-inducing activities. Phytomedicine. 2011, 18(8-9):748-755. (IF 2.662)

He XM, Ji N, Xiang XC, Luo P, Bao JK. Purification, characterization, and molecular cloning of a novel antifungal lectin from the roots of Ophioglossum pedunculosum. Applied Biochemistry and Biotechnology. 2011. 165(7-8):1458-1472. (IF 1.879)

Yu QJ, Li ZY, Yao S, Ming M, Wang SY, Liu B, Bao JK. In silico analysis of molecular mechanisms of Galanthus nivalis agglutinin-related lectin-induced cancer cell death from carbohydrate-binding motif evolution hypothesis. Applied
Biochemistry and Biotechnology. 2011. 165(3-4):1037-1046. (IF 1.879)

Zhang F, Li CY, Wang EQ, Li WW, Yu QJ, Wen X, Liu JJ, Bao JK*. The Global Component-Target Network in Panax Ginseng C. Letters in Drug Design & Discovery, 2011, 8, 181-188. (IF 0.668)

2010年

Liu B, Wu JM, Li J, Liu JJ, Li WW, Li CY, Xu HL, Bao JK*. Polygonatum cyrtonema lectin induces murine fibrosarcoma L929 cell apoptosis and autophagy via blocking Ras-Raf and PI3K-Akt signaling pathways. Biochimie. 2010. 92(12):1934-1938. (IF 4.864) 總引次數(shù)7次，他引次數(shù)1次。

Liu B, Bian HJ, Bao JK*. Plant lectins: Potential antineoplastic drugs from bench to clinic. Cancer Letters. 2010. 287(1):1-12. (IF 4.864) 總引用次數(shù)32，他引次數(shù)20次。

Yao Q, Wu CF, Luo P, Xiang XC, Liu JJ, Mou L, Bao JK*. A new chitin-binding lectin from rhizome of Setcreasea purpurea with antifungal, antiviral and apoptosis-inducing activities. Process Biochemistry. 2010. 45(9): 1477-1485. (IF: 2.648) 總引次數(shù) 4 次，他引次數(shù) 2 次。

Zhang ZT, Peng H, Li CY, Liu JJ, Zhou TT, Yan YF, Li Y, Bao JK*. Polygonatum cyrtonema lectin induces murine fibrosarcoma L929 cell apoptosis via a caspase-dependent pathway as compared to Ophiopogon japonicus lectin. Phytomedicine. 2010. 18(1):25-31. (IF 2.662) 總引次數(shù) 5 次，他引次數(shù) 1 次。

Li CY, Xu HL, Liu B, Bao JK*. Concanavalin A, from an old protein to novel candidate antineoplastic drug. Current Molecular Pharmacology. 2010. 3(3):123-128. EI文章 總引次數(shù) 6 次，他引次數(shù) 1 次。

Liu B, Cheng Y, Liu Q, Bao JK, Yang JM. Autophagic pathways as new targets for cancer drug development. Acta Pharmacologica Sinica. 2010. 31(9):1154-64. (IF 1.909) 總引次數(shù) 12 次，他引次數(shù) 4 次。

Ding J, Bao J, Zhu D, Zhang Y, Wang DC. Crystal structures of a novel anti-HIV mannose-binding lectin from Polygonatum cyrtonema Hua with unique ligand-binding property and super-structure. Journal of Structural Biology. 2010. 171(3):309-
17. (IF 3.497) 總引次數(shù) 4 次，他引次數(shù) 1 次。

2009年

Liu B, Min MW, Bao JK*. Induction of apoptosis by Concanavalin A and its molecular mechanisms in cancer cells. Autophagy, 2009. 5(3): 432-433. (IF 6.643) 總引次數(shù) 19 次，他引次數(shù) 6 次。

Liu B, Cheng Y, Bian HJ, Bao JK*. Molecular mechanisms of Polygonatum cyrtonema lectin-induced apoptosis and autophagy in cancer cells. Autophagy. 2009. 5(2):253-255. (IF 6.643) 總引次數(shù) 26 次，他引次數(shù) 11 次。

Liu B, Cheng Y, Zhang B, Bian HJ, Bao JK*. Polygonatum cyrtonema lectin induces apoptosis and autophagy in human melanoma A375 cells through a mitochondria-mediated ROS-p38-p53 pathway. Cancer Letters. 2009. 275(1):54-60. (IF 4.864) 總引次數(shù) 45 次，他引次數(shù) 25 次。

Liu B, Li CY, Bian HJ, Min MW, Chen LF, Bao JK*. Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells. Archives of Biochemistry and Biophysics. 2009. 482(1-2):1-6 (IF 3.022) 總引次數(shù) 24 次，他引次數(shù) 11 次。

Liu B, Zhang B, Min MW, Bian HJ, Chen LF, Liu Q, Bao JK*. Induction of apoptosis by Polygonatum odoratum lectin and its molecular mechanisms in murine fibrosarcoma L929 cells. Biochimica et Biophysica Acta (BBA) - General Subjects. 2009. 1790(8):840-844. ( IF 4.663) 總引次數(shù) 19 次，他引次數(shù) 6 次。

Peng H, Lv H, Wang Y, Liu YH, Li CY, Meng L, Chen F, Bao JK*. Clematis montana lectin, a novel mannose-binding lectin from traditional Chinese medicine with antiviral and apoptosis-inducing activities. Peptides. 2009. 30(10):1805-1815. (IF 2.654) 總引次數(shù) 12 次，他引次數(shù) 9 次。

Lv H, Kong Y, Yao Q, Zhang B, Leng FW, Bian HJ, Balzarini J, Van Damme E, Bao JK*. Nebrodeolysin, a novel hemolytic protein from mushroom Pleurotus nebrodensis with apoptosis-inducing and anti-HIV-1 activities. Phytomedicine. 2009. 16(2-3):198-205. (IF 2.662) 總引用次數(shù)7次，他引次數(shù)4次。

Chen J, Liu B, Ji N, Zhou J, Bian HJ, Li CY, Chen F, Bao JK*. A novel sialic acid-specific lectin from Phaseolus coccineus seeds with potent antineoplastic and antifungal activities. Phytomedicine. 2009. 16(4):352-360. (IF 2.662) 總引用次數(shù)23次，他引次數(shù)15次。

Liu B, Peng H, Yao Q, Li J, Van Damme E, Balzarini J, Bao JK*. Bioinformatics analyses of the mannose-binding lectins from Polygonatum cyrtonema, Ophiopogon japonicus and Liparis noversa with antiproliferative and apoptosis-inducing activities. Phytomedicine. 2009. 16(6-7):601-608. (IF 2.662) 總引用次數(shù)15次，他引次數(shù)3次。

Ye SH, Chen S, Zhang F, Wang W, Tian Q, Liu JZ, Chen F, Bao JK*. Transgenic tobacco expressing Zephyranthes grandiflora agglutinin confers enhanced resistance to aphids. Applied Biochemistry and Biotechnology. 2009. 158(3):615-30. (IF 1.879) 總引用次數(shù)10次，他引次數(shù)9次。

Li CY, Wang Y, Peng H, Bian HJ, Min MW, Chen LF, Liu Q, Bao JK*. In silico Analysis of the Potential Infection Mechanisms of Magnaporthe grisea from Horizontal Gene Transfer Hypothesis. Genomics, Proteomics & Bioinformatics. 2009. 7(3):77-86.
Li CY, Meng L, Liu B, Bao JK*. Galanthus nivalis agglutinin (GNA)-related lectins: Traditional proteins, Burgeoning drugs? Current Chemical Biology. 2009. 3:324-333.

EI 總引次數(shù) 9 次，他引次數(shù) 1 次。

Zhao Q, Wu CF, Wang WG, Yuan S, Bao JK, Chen F. In vitro plantlet regeneration system from rhizomes and mannose-binding lectin analysis of Polygonatum cyrtonema Hua. Plant Cell, Tissue and Organ Culture. 2009. 99(3): 269-275. (IF 1.243)

2008年

Liu B, Xu XC, Cheng Y, Huang J, Liu YH, Liu Z, Min MW, Bian HJ, Chen J, Bao JK*. Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema lectin and chemical modification properties on its mannose-binding sites. BMB Reports. 2008. 41(5):369-375. (IF 2.167) 總引用次數(shù)24次，他引次數(shù)1次。

Liu C, Zhao X, Xu XC, Li LR, Liu YH, Zhong SD, Bao JK*. Hemagglutinating activity and conformation of a lactose-binding lectin from mushroom Agrocybe cylindracea. International Journal of Biological Macromolecules. 2008. 42(2):138-144. (IF 2.502) 總引用次數(shù)6次，他引次數(shù)4次。

Liu Z, Liu B, Zhang ZT, Zhou TT, Bian HJ, Min MW, Liu YH, Chen J, Bao JK*. A mannose-binding lectin from Sophora flavescens induces apoptosis in HeLa cells. Phytomedicine. 2008. 15(10):867-875. (IF 2.662) 總引用次數(shù)31次，他引次數(shù)16次。

Tian Q, Wang W, Miao C, Peng H, Liu B, Leng FW, Dai L, Chen F, Bao JK*. Purification, characterization and molecular cloning of a novel mannose-binding lectin from rhizomes of Ophiopogon japonicus with antiviral and antifungal activities. Plant Science. 2008. 175(6): 877-884. (IF 2.481) 總引次數(shù) 13 次，他引次數(shù) 4 次。

Xu XC, Zhong SD, Kai F, Li LR, Liu C, Liu B, Bao JK*. Preparation and characterization of a novel recombinant human parathyroid hormone (1-34) analog (Gly1-Gln26-rhPTH(1-34)) with enhanced biological activity. Protein & Peptide Letters. 2008. 15(8):854-860. (IF 1.849) 總引次數(shù) 0 次，他引次數(shù) 0 次。

Ding JJ, Bao JK, Zhu DY, Zhang Y, Wang DC. Crystallization and Preliminary X-Ray Diffraction Analysis of a Novel Mannose-Binding Lectin with Antiretroviral Properties from Polygonatum cyrtonema Hua. Protein and Peptide Letters. 2008. 15(4):411-414. (IF 1.849) 總引次數(shù) 4 次，他引次數(shù) 1 次。

2007年

Luo YT, Xu XC, Liu JW, Li J, Sun YS, Liu Z, Liu JZ, Van Damme E, Balzarini J, Bao JK*. A novel mannose-binding tuber lectin from Typhonium divaricatum (L.) Decne (family Araceae) with antiviral activity against HSV-II and anti-proliferative effect on human cancer cell lines. Journal of Biochemistry and Molecular Biology. 2007. 40(3):358-367. (IF 2.021) 總引用次數(shù)23次，他引次數(shù)14次。

Liu JW, Xu XC, Liu JZ, Balzarini J, Luo YT, Kong Y, Li J, Chen F, Van Damme E, Bao JK*. A novel tetrameric lectin from Lycoris aurea with four mannose-binding sites permonomer. Acta Biochimica Polonica. 2007. 54(1):159-166. (IF 1.234) 總引用次數(shù)6次，他引次數(shù)3次

Xu XC, Wu CF, Liu C, Luo YT, Li J, Zhao XP, Damme EV, Bao JK*. Purification and Characterization of a Mannose-Binding Lectin from the Rhizomes of Aspidistra elatior Blume with Antiproliferative Activity. Acta Biochimica et Biophysica Sinica (Shanghai). 2007. 39(7): 507-519 (IF 1.547) 總引用次數(shù)14次，他引12次

學術專著

Huai-long Xu, Lei-lei Fu, Yu Yang, Bo Liu, Xu Zhao, Wen-Wen Li, Jin-Ku Bao. Autophagy pathways and drug targets in cancer. In: Autophagy: Principles, Regulation and Roles in Disease. Accepted for Publication. August, 2011. Nova Science Publisher. Hauppauge NY, USA。

鮑錦庫，劉博，《自噬：生物學與疾病》第二十八章：針對自噬的藥物開發(fā)和治療的展望。2011年3月 科學出版社。

其它論著

An J, Liu JZ, Wu CF, Li J, Dai L, Van Damme E, Balzarini J, De Clercq E, Chen F, Bao JK*. Anti-HIV I/II activity and molecular cloning of a novel mannose/sialic acid-binding lectin from rhizome of Polygonatum cyrtonema Hua. Acta Biochimica et Biophysica Sinica (Shanghai). 2006. 38(2):70-8. (IF 1.547) 總引用次數(shù)26次，他引次數(shù)8次。

Wu CF, Li J, An J, Chang LQ, Chen F, Bao JK*. Purification, Biological Activities, and Molecular Cloning of a Novel Mannose-Binding Lectin from Bulbs of Zephyranthes candida Herb (Amaryllidaceae). Journal of Integrative Plant Biology. 2006. 48(2):223-231. (IF 1.603)

Gao S, An J, Wu CF, Gu Y, Chen F, Yu Y, Wu QQ, Bao JK*. Effect of amino acid residue and oligosaccharide chain chemical modifications on spectral and hemagglutinating activity of Millettia dielsiana Harms. ex Diels. Lectin. Acta Biochimica et Biophysica Sinica (Shanghai). 2005. 37(1): 47-54. (IF 1.547)

Gong M, An J, Lü HZ, Wu CF, Li YJ, Cheng JQ, Bao JK*. Effects of denaturation and amino acid modification on fluorescence spectrum and hemagglutinating activity of Hericium erinaceum Lectin. Acta Biochimica et Biophysica Sinica (Shanghai). 2004. 36(5):343-350 (IF 1.547)

Wu CF, An J, He XJ, Deng J, Hong ZX, Liu Chao, Lu HZ, Li YJ, Wang CJ, Chen F, Bao JK*. Molecular Cloning of a Novel Mannose-binding Lectin Gene from Bulbs of Amaryllis vittata (Amaryllidaceae). Acta Botanica Sinica. 2004. 46(11):1301-1306.

Jiang FB, Du J, Yu XQ, Bao JK, Zeng XC. Metallomicellar catalysis: accelerated hydrolysis of BNPP by copper(II), zinc(II), and nickel(II) complexes of long alkanol-imidazole in CTAB micellar solution. Journal of Colloid and Interface Science. 2004. 273(2):497-504. (IF 3.066)

Cai YF, Zhang H, Zeng Y, Mo JC, Bao JK, Miao C, Bai J, Yan F, Chen F. An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton. Journal of Biosciences. 2004. 29(1):67-71. (IF 1.888)

Biological Activity Analysis of Polygonatum cyrtonema Hua. Lectin by Site-Directed Mutagenesis, Trends in Glycoscience and Glycotechnology, 2004,16:pp.s1–s32（IF 0.863）

In Vitro Plantlet Regeneration from Rhizome Lumps and Mannose-Binding Lectin Analysis of Polygonatum cyrtonema Hua.(Family Liliaceae) ,Trends in Glycoscience and Glycotechnology(TIGG), 2004,16: pp.S 33–S 64（IF 0.863）

Miao C, Gou XP, Lan LQ, Bao JK, Xu Y, Wang SH, Chen F. Molecular cloning and expression of RSSSG58 gene in rice sperm cells. Acta Botanica Sinica. 2003. 45(2):234-241.

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國家發(fā)明專利：

1、一種大量獲得黃精凝集素II的方法。專利號：ZL 2004 1 0021659.1

2、黃精凝集素Ⅱ蛋白在制備治療或預防乙型肝炎的藥中的應用。專利號：ZL 2004 1 0022354.2

3、黃精凝集素II蛋白在制備治療或預防單純皰疹病毒所致疾病的藥中的應用。專利號：ZL 2004 1 0022353.8

4、黃精凝集素II蛋白在制備治療或預防艾滋病的藥中的應用。專利號：ZL 2006 1 0005577.7