華中農業大學園藝林學學院林學與茶學系導師介紹：寧國貴

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寧國貴：男，1979年1月生，博士。華中農業大學副教授,碩士生導師。博士畢業論文題目為“矮牽牛不同花型突變體的形態結構及其分子基礎分析”。主要研究領域：園林植物細胞與分子生物學。目前從事的研究領域具體涉及到(1)野薔薇、月季和玫瑰等（薔薇科Rosaceae）等木本植物(2)矮牽牛和煙草等（茄科Solanaceae）等草本植物的開花時間調控及開花過程中花色、花香等次生代謝物代謝方面的相關分子生物學研究。主要研究方法：生物信息分析與比較基因組，轉基因分析重要功能候選基因，液相氣質等測定分析代謝物等的研究方法。已在國內外發表論文近30篇。

Email: ggning@mail.hzau.edu.cn & whnobleman2004@yahoo.com.cn

TEL: 　027-87286928 (o)

主講課程如下：

《園林植物育種學》、《花卉學》、《園林植物育種學實驗》、《花卉學實驗》、《園藝植物育種學》等課程。

主持與參加主要科研項目如下：

1.瑞典國際科學基金：Shorting juvenility of Platanus acerifolia and Prunus mume via transformation using FT orthologs（D/4349-1，項目負責人）2.國家自然科學基金：梅花開花時間相關基因的克隆及其功能解析（30800761，項目負責人）

3.國家自然科學基金： 月季及其近緣種MYB 不同類型轉錄因子的結構及功能研究（31171985，項目負責人）

4.科技部“十二五”863基金：月季、菊花、百合分子育種與品種創制（2011AA100208，月季子項目分子育種部分負責人）

5.華中農業大學科技創新基金：薔薇屬三物種花發育代謝物質的差異性及其與酶基因的相關性研究(2011PY103，項目負責人）

6.國家自然科學基金：早花變異枳LFY基因啟動子結構及表達機制調控研究(30671434, 項目參加人)

7.國家自然科學基金項目：矮牽牛花器官發育的分子遺傳分析及人工調控(30671477, 項目參加人)

8.教育部博士點基金：表皮毛發育相關基因對煙草與矮牽牛花青素含量調控的機理研究(2008-2010，項目參加人)

已發表的主要論文：

1.Guogui Ning*, Xiao Xiao, Haiyan Lv, Xin Li,Yang Zuo, Manzhu Bao*.Shortening tobacco life-cycle accelerates functional gene identification in genomic research. Plant biology 2012, In press (SCI)

2.Liao Liao, Cai-Xian Liu, ·Guo-Gui Ning,· Man-Zhu Bao. A pFBP6::BARNASE construct resulted in stigma and style ablation and floral abscission in transgenic tobacco. Plant molecular biology reporter 2012, In press (SCI)

3.Zhen Wang, Shafei Ye, Jingjing Li, Bo Zheng, Manzhu Bao, Guogui Ning*.Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning. BMC Biotechnology 2011, 11:109 doi: 10.1186/1472-6750-11-109(SCI)

4.Ying Bao Guofeng Liu Xueping Shi Wen Xing Guogui Ning Juan Liu Manzhu Bao Primary and repetitive secondary somatic embryogenesis in Rosa hybrida ‘Samantha’Plant cell, tissue &organ culture. 2011, In press (SCI)

5.Shijia Luo, Yanhong He, Guogui Ning, Jiaqi Zhang, Guangying Ma and Manzhu Bao. Genetic diversity and genetic structure of different populations of the endangered species Davidia involucrata in China detected by inter-simple sequence repeat analysis. Trees,2011, DOI: 10.1007/s00468-011-0581-7(SCI)

6.J. Zhang, G. Liu, C. Guo, Y. He, Z. Li, G. Ning, X. Shi & M. Bao. The FLOWERING LOCUS T orthologous gene of Platanus acerifolia is expressed as alternatively spliced forms with distinct spatial and temporal patterns. 2010, doi:10.1111/j.1438-8677.2010.00432.x(SCI)

7.Yanhong He,Guogui Ning*, Yalin Sun, Yanhu, Xingyu Zhao, Manzhu Bao. Cytological and mapping analysis of a novel male sterile type resulting from spontaneous floral organ homeoticconversion in marigold (Tagetes erecta L.). Mol Breeding,2010,DOI 10.1007/s11032-009-9372-x(SCI)

8.Wen Xing, Manzhu Bao, Hongdao Qin,Guogui Ning*. Micropropagation of Rosa Rugosa through axillary shoot proliferation.ACTA BIOLOGICA CRACOVIENSIA Series Botanica 2010,52/2: 75–56(SCI)

9.Shi XP, Dai XG, Liu GF, Zhang JW, Ning GG, Bao MZ. Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.). In Vitro Cell Dev Biol Plant, 2010, DOI 10.1007/s11627-009-9272-0(SCI)

10.Guangying Ma, Guogui Ning, RuihuaPang, JingZhan, XinLi,WeiZhang, Manzhu Bao. Analysis ofthe Petunia hybrida double flowertranscriptomeusingsuppression subtractive hybridization. Scientia Horticulturae ，doi:10.1016/j.scienta.2010.10.003(SCI)

11.Guangying Ma, Guogui Ning, Wei Zhang, Jing Zhan, Haiyan Lv, Manzhu Bao.Overexpression of Petunia SOC1-like Gene FBP21 in Tobacco Promotes Flowering Without Decreasing Flower or Fruit Quantity. Plant Mol Biol Rep, 2010,DOI 10.1007/s11105-010-0263-4(SCI)

12.Wei Zhang, Guogui Ning, Haiyan Lv, Liao Liao, Manzhu Bao. Single MYB-type transcription factor AtCAPRICE: A new efficient tool to engineer the production of anthocyanin in tobacco. Biochemical and Biophysical Research Communications, 2009,388(4):742-747(SCI)

13.Y.H. He, G.G. Ning, Y.L. Sun, Y.C. Qi and M.Z. Bao.Identification of a SCAR marker linked to a recessive male sterile gene(Tems) and its application in breeding of marigold (Tagetes erecta L.). plant breeding,2009, 128: 92—96 (SCI)

14.G.G.Ning, X.P. Shi, H.R Hu, Y.Yan, M.Z. Bao. .Development of a range of polyploid lines in Petunia hibrida and the relationship of ploidy with the single-/ double-flower trait. Hortscience, 2009,44(2):250-255(SCI)

15.W.J. Huang, G.G. Ning, G.F. Liu, M.Z. Bao. Determination of genetic stability of long-term micro-propagated plantlets of Platanus acerifolia using ISSR markers. BIOLOGIA PLANTARUM ,2009,(1): 159-163 (SCI)

16.XiaoPeng Fu, GuoGui Ning, LiPing Gao, ManZhu Bao .Genetic diversity of Dianthus accessions based on molecular marker systems (SRAPs and ISSRs) and morphological traits. Scientia Horticulturae, 2008,113:263-270 (SCI)

17.Y. M. Ye J. W. Zhang G. G. Ning & M. Z. Bao. A comparative analysis of the genetic diversity between inbred lines of Zinnia elegans using morphological traits and RAPD and ISSR markers. Scientia Horticulturae, 2008,118:1–7(SCI)

18.G. G Ning, S. P Bai, M. Z Bao, L. Liu. Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume ‘Xue Mei'. In Vitro Cell. Dev. Biol.-Plant. . 2007, 43: 95-100(SCI)

19.G.G Ning, M.Z Bao.Plant regeneration from callus derived from immature embryo cotyledons of Prunus mume. HortScience, 2007, 42(3): 744-747(SCI)

20.G.G NING, X.L FAN, W.J HUANG, M.Z BAO, J.B. Micropropagation of six Prunus mume cultivars through high-frequency axillary shoots proliferation and ISSR analysis of cloned plants. Acta Biologica Cracoviensia, 2007,49(1):25-31(SCI)

21.包穎，張蔚，邢文，寧國貴，包滿珠* MYB家族基因CPC在月季中的遺傳轉化研究. 中國科技論文在線,2012,http://www.paper.edu.cn/index.php/default/releasepaper/content/201201-763

22.邢文,張蔚,包穎,寧國貴,包滿珠*植物類黃酮生物合成的MYB轉錄因子研究進展.中國科技論文在線，2012,http://www.paper.edu.cn/index.php/default/releasepaper/content/201201-789

23.寧國貴，呂海燕，張俊衛，包滿珠.梅花不同外植體離體培養及體細胞胚誘導植株再生. 園 藝學 報 2010， 1：114 – 120

24.王文恩,包滿珠,張俊衛,劉國鋒,寧國貴,尹少華.狗牙根輻射誘變后代變異植株的形態特征比較和ISSR分析. 草業科學，2009，26(12):139-145

25.呂海燕,寧國貴,胡惠蓉,包滿珠.矮牽牛突變自交系離體培養及變異植株穩定性觀察. 華中農業大學學報，2009 ,28(6):746～749

26.寧國貴，白三平，包滿珠，黃文俊.矮牽牛細胞的長期離體培養及再生植株的 ISSR 分析. 中國農業科學.2007,40(7):1479-1485

27.齊迎春,寧國貴,包滿珠.應用 ISSR 分子標記和表型性狀評價孔雀草自交系的遺傳關系. 中國農業科學.2007,40(6): 1236-1241

28.寧國貴,包滿珠.矮牽牛一種新花器官突變體的表型與解剖結構特征分析.園藝學報,2006,5:1121-1124

29.寧國貴，代色平，包滿珠，沈寶仙. 矮牽牛S4代13個自交系主要性狀分析中南林學院學報，2003， 23（5）：76－79

目前主要專利申請：

1.農桿菌介導的玫瑰遺傳轉化方法（排名第一）

2.一種獲得月季‘薩曼莎’轉基因植株的方法（排名第一）

3.玫瑰再生為完整植株的方法（排名第一）

4.以未成熟種子為外植體的月季再生植株的方法（排名第五）

5.以葉片為外植體的月季再生植株的方法（排名第五）